THE EFFECTIVENESS TEST OF PULP MACERATION (Mimusops elengi L) ON BACTERIAL GROWTH NORMAL MICROFLORAS OF MOUTH

Abstract

Tanjung or Mimusops elengi L. tree is a member of the Sapotaceae tribe which is known as a evergreen tree. So far, the headland has only been used as a shade planted in parks and on the side of the road, even though the headland has a number of health benefits. Flora in the oral cavity basically has a harmonious relationship with the host and consists of a variety of organisms, including bacteria, fungi, mycoplasma, protozoa, and viruses that can be encountered from time to time. The research objective was to determine whether the ethanolic extract of Tanjung (Mimusops elengi L) fruit had anti-bacterial inhibition against oral microflora. This research was a true-experiment which aims to determine the possible cause and relationship effect of one or more groups being studied. The research was carried out in several locations of Tanjung tree plants in Medan city, and secondary metabolite testing (Phytochemical Screening) was carried out at the Pharmacy Laboratory of North Sumatra university (USU) as well as at the Microbiology Laboratory of Poltekkes Medan. The research time started after receiving the title of the proposal until the final report. Improvement of the proposal until completion was estimated from November 2019 to July 2020. The population was cape plants in the city of Medan, and samples are taken from fruiting and ripe cape plants (red fruit) in the Medan Estate area. In the test phase for the extract's antibacterial activity, the inhibitory diameter data will be obtained for each of the tested bacteria at a certain concentration. Data were analyzed using the One Way ANOVA statistical test. Before the test was carried out, the normality test was carried out first to ensure that the data were normally distributed. In the 25% extract treatment, 50% of Tanjung fruit pulp against Staphylococcus aureus and Streptococcus mutans showed p value <0.05 and 100% Tanjung fruit pulp extract against Streptococcus mutans bacteria showed p value <0.05. Where these results indicated the data was not normally distributed so that the Kruskal Wallis test was continued. The results of post-hoc Mann Whitney analysis showed that there were differences in the growth inhibition zone of Staphylococcus aureus bacteria, Lactobacillus acidophilus, Streptococcus mutans between levels of concentration and also had significant differences between treatments in all treatments at various levels of concentration (p <0.05) Tanjung fruit flesh. The zone of inhibition of growth of Staphylococcus aureus, Lactobacillus acidophilus, Streptococcus mutans bacteria between concentration levels also had a significant difference (p <0.05). So that the effect of different concentrations on the diameter of inhibition produced by the etanolic extract of Tanjung fruit pulp (Mimusops elengi L) against normal micro bacteria in mouth. Tanjung fruit pulp extract can inhibit the growth of Lactobacillus acidophilus, Staphylococcus aureus and Streptococcus mutans bacteria at test concentrations of 25%, 50%, 75% and 100%. The most effective concentration which has a diameter of the inhibition zone was a concentration of 100% with an average diameter of the inhibition zone formed in each of Lactobacillus acidophilus bacteria of 27.925 mm, Staphylococcus aureus of 9.425 mm and Streptococcus mutans of 14.825 mm. The extract of Tanjung fruit pulp was more effective for inhibiting the growth of Lactobacillus acidophilus bacteria. Keywords: Inhibition, Tanjung Fruit (Mimusops elengi L), Normal Microflora in Mouthrite abstract here (Time New Roman 10, Regular). The rule of abstract writing are: 1) maximum: 300 words, 2) without indentation of paragraph, 3) with 2-6 keywods in the form of word or phrase.
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